エフシージー総合研究所・フジテレビ商品研究所

2011年論文発表

Allergenicity and Cross-Reactivity of Booklice (Liposcelis bostrichophila) : A Common Household Insect Pest in Japan

Yuma Fukutomi, Yuji Kawakami, Masami Taniguchi, Akemi Saito, Azumi Fukuda, Hiroshi Yasueda, Takuya Nakazawa, Maki Hasegawa, Hiroyuki Nakamura, Kazuo Akiyama

Background: Booklice (Liposcelis bostrichophila) are a common household insect pest distributed worldwide. Particularly in Japan, they infest ‘tatami’ mats and are the most frequently detected insect among all detectable insects, present at a frequency of about 90% in dust samples. Although it has been hypothesized that they are an important indoor allergen, studies on their allergenicity have been limited.
Methods: To clarify the allergenicity of booklice and the cross-reactivity of this insect allergen with allergens of other insects, patients sensitized to booklice were identified from 185 Japanese adults with allergic asthma using skin tests and IgE-ELISA. IgE-inhibition analysis, immunoblotting and immunoblotting- inhibition analysis were performed using sera from these patients. Allergenic proteins contributing to specific sensitization to booklice were identified by two-dimensional electrophoresis and two-dimensional immunoblotting.
Results: The booklouse-specific IgE antibody was detected in sera from 41 patients (22% of studied patients). IgE inhibition analysis revealed that IgE reactivity to the booklouse allergen in the sera from one third of booklousesensitized patients was not inhibited by preincubation with extracts from any other environmental insects in this study. Immunoblotting identified a 26-kD protein from booklouse extract as the allergenic protein contributing to specific sensitization to booklice. The amino acid sequence of peptide fragments of this protein showed no homology to those of previously described allergenic proteins, indicating that this protein is a new allergen. Conclusions: Sensitization to booklice was relatively common and specific sensitization to this insect not related to insect panallergy was indicated in this population.

環境科学研究室では、「ダニ」「カビ」「微小昆虫類」「抗菌性評価」など
室内環境の有害生物の研究・調査に対応いたします。

【室内環境生物分野の試験および研究】
  • 室内環境中で健康被害を及ぼすダニ・微小昆虫類・カビなどの研究
  • 喘息やアトピー性皮膚炎などのアレルゲンとなる生物の研究
  • 大学や国公立の研究機関との共同研究
  • 空気清浄機の除菌性能評価、抗菌素材の性能評価などの商品実用試験
  • 異物混入検査

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